Sintesis Interleukin 1ß Makrofag Mencit yang Diinduksi Lipopolisakarida E. coli dan Minyak Atsiri Kencur

Abstract

The purpose of this study was to evaluate the effectiveness of the essential oil of Kaempheria galangal as an anti-inflammatory agent, by evaluating its effect on interleukein 1ß (IL-1ß) produced by lipoplysaccharide (LPS) E. coli stimulated macrophages. Macrophage cells were stimulated with LPS E. coli and essential oil of Kaempheria galangal at concentration of 3µl/ml, 1.5 µl/ml, 0.75 µl/ml and 0.25 µl/ml. All of the treatment groups were incubated at 37ºC in an atmosphere containing 5% CO2 for 24 hours. The concentrations of IL-1ß supernatant were determined by using an enzyme-linked immunosorbent assay kit. Statistical analysis using ANOVA showed significant differences between the treatment groups (p<0.05). This study indicates that the essential oil of Kaempheria galangal suppressed IL-1ß produced by LPS E. coli stimulated macrophage cells.