Reduction in matrix-related signal suppression effects in electrospray ionization mass spectrometry using on-line two-dimensional liquid chromatography

Abstract

The effect of liquid chromatographic separation on matrix-related signal suppression in electrospray ionization mass spectrometry (LC - ESI-MS) was investigated. A method incorporating on-line two-dimensional liquid chromatography mass spectrometry (LC/LC - MS) was developed to compensate for matrix effects and signal suppression in qualitafive and quanfitafive analysis. The LC/LC - MS(MS) approach was successfully applied for single-component and multicomponent analysis in a variety of complex matrixes. It was demonstrated that matrix-related signal suppression could be induced solely by (i) column overload, (ii) matrix component-analyte coelution, or a combination of each. Application of on-line orthogonal LC/LC separadons can be effective in reducing both causes of matrix - related signal suppression effects i.e., column overload and matrix - analyte coelution for a variety of LCn/MSn applications.