Genetic diversity of Shorea producing tengkawang populations in Kalimantan based on SSR markers

Abstract

Shorea producing tengkawang grows naturally in Indonesia, however several species are now listed in the IUCN as endangered species. Their presences in natural forests arebecoming scarce so that it requires conservation efforts such as the construction of ex-situ conservation plots. This research aims to obtain data and information about the genetic diversity structure of several species of Shorea producing tengkawang. The material used was leaf samples from 11 (eleven) populations consisting of 4 (four) species of Shorea producing tengkawang. The method used 4 pairs of microsatellite DNA markers (SSR), and data analysis used POPGENE and GenAleX. The results show various genetic distances between populations, ranging from 0.028 to 1.510 with the value of genetic diversity in the population larger (72%) than among the populations (28%). Genetic diversity in the population ranges from 0.5269-0.8244. Cluster analysis shows that the existing Shorea producing Tengkawang population does not form a specific grouping. This genetic diversity information can be used as the basic data for the development of Shorea producing tengkawang ex-situ conservation plots.