Structure elucidation of acylsucrose derivatives from Atractylodes lanceae rhizome and Atractylodes rhizome

Abstract

The mass chromatographic fingerprints of the water extracts of Atractylodes rhizome and Atractylodes lancea rhizome were analyzed by LC-MS. Three new acylsucrose derivatives [2,6,3′,6′-tetra(3-methylbutanoyl)sucrose (1), 2,4,3′,6′-tetra(3-methylbutanoyl)sucrose (2), and a mixture of 3′,4′,6′-tris(3-methylbutanoyl)-1′-(2-methylbutanoyl) sucrose and 1′,3′,4′,6′-tetra(3-methylbutanoyl)sucrose (6)), along with three known acylsucroses (2,6,3′,4′-tetra(3- methylbutanoyl)sucrose (3), 2,4,3′,4′-tetra(3-methylbutanoyl)sucrose (4) and a mixture of 2,3′,6′-tris(3-methylbutanoyl)-1′-(2- methylbutanoyl)sucrose and 2,1′,3′,6′-tetra(3-methylbutanoyl) sucrose (5)] were isolated as the marker compounds of Atractylodes rhizome and Atractylodes lancea rhizome. The structures of the compounds were elucidated by MS/MS analyses and NMR experiments.