Freezing rabbit semen by the use of BF5 diluent

Abstract

Three experiments were carried out to find the optimal concentration of DMSO and glycerol in BFS diluent for freezing rabbit spermatozoa. Semen was diluted 1:1 with diluent A (BF5 + DMSO) at 25°C and diluted further 1:1 with diluent B (diluent A + glycerol) after cooling down to 5°C. Diluted semen was frozen immediately and stored in liquid nitrogen. Maximum percentages for motility and normal acrosomes were obtained in the presence of 12% DMSO (as expressed in diluent A) and 3% glycerol (final concentration) after thawing. © 1990, Royal Society of Medicine Press. All rights reserved.