Evaluation of the antityrosinase and antioxidant potential of zinc oxide nanoparticles synthesized from the brown seaweed-Turbinaria conoides

Abstract

Objective: The present study was designed to evaluate the antioxidant and antityrosinase properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from brown seaweed Turbinaria conoides. Methods: Zinc Oxide Nanoparticles were synthesized from the hydroethanolic extract of Turbinaria conoides. Ultraviolet-Visible Spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology and elemental composition of ZnO-NPs were analysed using SEM-EDAX. The antioxidant activity of the synthesized zinc oxide nanoparticles was investigated by total antioxidant capacity (phosphomolybdenum method), reducing power assay and ferric reducing antioxidant power assay (FRAP). Anti tyrosinase activity was assessed to validate the skin whitening ability of the ZnO-NPs. Results: The antioxidant activity of ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides was maximum when compared with that of the hydroethanolic algal extract. The antityrosinase activity of ZnO-NPs was found to be maximum with 75% tyrosinase inhibition when compared to hydroethanolic algal extract which had 56% inhibition at 250μg/ml concentration. Conclusion: Overall our study provides a firm evidence to support that antityrosinase and antioxidant activities are exhibited by ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides and it might be used as an antioxidant and as a source of skin whitening agent in cosmetics.