Crystallization and preliminary x-ray analysis of the major cellulose-binding domain of the cellulosome from clostridium thermocellum

Abstract

The cellulose-binding domain from the scaffoldin subunit of the cellulosome from Clostridium thermocellum strain YS has been expressed in Escherichia coli, purified to homogeneity, and crystallized. Crystals were grown by vapor diffusion using polyethylene glycol as precipitant. They belong to the monoclinic space group C2 with unit cell dimensions of a = 64.68 Å, b = 50.36 Å c =96.27 Å β = 99.43β, and density packing considerations suggest that the asymmetric unit contains two molecules. The crystals diffract beyond 2.0 Å resolution using a laboratory rotating anode source. © 1994 Academic Press Limited.