Reversed-Phase Liquid Chromatography of Basic Drugs and Pesticides with a Fluorigenic Ion-Pair Extraction Detector

Abstract

An ion-pair extraction detector for the sensitive and selective fluorescence detection of some basic drugs, pesticides, and their metabolites is described. The addition of the ion-pairing reagent dimethoxyanthracene sulfonate (DAS) prior to the column permits a drastic simplification of the detector design. The influence of the DAS concentration, pH, buffer concentration, and organic polarity modifier (methanol) on the separation of the above compounds carried out in the reversed-phase mode on various chemically bonded materials has been studied. The flexibility of the system is such that the mo-bile-phase composition can be attuned for optimal detector performance. The technique is finally applied to the trace determination of hydroxyatrazine in urine. © 1980, American Chemical Society. All rights reserved.