Primary cell culture from gills of striped bass morone saxatilis (Walbaum, 1792)

Abstract

Despite the relevance of primary cell culture from the gill tissue, there are no reports on the branchial cell lines of the striped bass Morone saxatilis (Walbaum, 1792). The importance of establishing cell lines from M. saxatilis branchial tissue represents an alternative to studying infective physiological and biochemical processes, which may facilitate research without compromising valuable live animals. Branchial tissue explants were cultured in 2 × Leibovitz L-15 (pH 7.2 ± 2) medium supplemented with 20 % foetal bovine serum (FBS) and 2 × antibiotic-antimycotic adjusted to 578 mOsm.kg-1. Primary cell cultures obtained showed excellent cell adhesion and proliferation. The cell monolayer consisted of epithelial cells, and the culture was maintained for approximately 30 days. This is the first report describing primary branchial cell line culture from striped bass.