CYP1A1 gene polymorphism in inflammatory and non inflammatory pterygium

Abstract

Background and Objective: Pathogenesis of pterygium is not fully understood. CYP1A1 gene found to have role in carcinogen metabolisms and also detected in pterygium tissue. The purpose of this study was to assess the CYP1A1 gene polymorphisms in patients with inflammatory and non-inflammatory pterygium. Methods: The PCR was conducted on blood samples of 80 patients with pterygium which consisted of 40 inflammatory and 40 non-inflammatory pterygium. The rs 4646903 SNP genotyping T>C (m1) in the CYP1A1 gene was conducted using Restriction Fragment Length Polymorphisms-PCR (RFLP-PCR). The PCR products were cut with restriction enzyme MspI. Results of restriction were then electrophorised and then observed with GelDoc. Results: In inflammatory pterygium, 13 patients obtained TT alleles (wild type), 19 TC allele (heterozygous mutants) and 8 CC alleles (homozygous mutant). In non-inflammatory pterygium, 14 patients obtained TT allele (wild type), 18 TC allele (heterozygous mutant) and 8 CC alleles (homozygous mutant). Conclusion: The CYP1A1 gene polymorphisms may occur in pterygium patients either inflammatory or non-inflammatory. Polymorphisms occur in the form of heterozygous and homozygous mutant.