Comparative Dynamics of DNA Isolated from Buccal Swab for Forensic Investigation “Pro and Post Brushing from Some Adult Males in Benin Metropolis, Nigeria”

Abstract

The present study was performed to determine the quality and the quantity of DNA extracted from buccal swab at two intervals to compare the DNA quality and quantity before and after brushing. Five (5) healthy males where used in the study, ten (10) Buccal swabs where collected at two intervals, five (5) before brushing and five (5) after brushing commonly available samples and to estimate the DNA yield and purity from the various times of collection. The purity and the concentration of the extracted DNA were determined spectrophotometric analysis, and the adequacy of DNA extracts for the PCR-based assay was assessed by amplifying a 1030-bp region of the mitochondrial D-loop. Although DNA from all the intervals was suitable for PCR, the samples before brushing when the mouth was empty provided a good quality DNA for restriction analysis of the PCR product compared with the buccal swab after brushing. In the present study, buccal swab samples showed to be a good source of genomic DNA for PCR-based methods.