Quantification of myocardial stiffness in heart failure with preserved ejection fraction porcine model using magnetic resonance elastography

Abstract

Background: Heart failure with preserved ejection fraction (HFpEF) is associated with a complex heterogeneous pathophysiology which is poorly understood thereby preventing appropriate diagnosis and prognosis1. However, it is known that most of the cardiovascular and non-cardiac abnormalities that induce HFpEF are eventually manifested as an increase in left ventricular (LV) myocardial stiffness (MS). Therefore, we hypothesize that quantifying MS will assist in timely diagnosis of HFpEF and reveal pathophysiological conditions that are specific to the prognosis of HFpEF. Recently, with the advent of cardiac magnetic resonance elastography (cMRE) it has been feasible to estimate the shear stiffness of myocardium noninvasively2. In this study, we use cMRE to estimate the change in LV MS across the cardiac cycle during disease progression in HFpEF induced pigs. Methods: Renal wrapping surgery was performed in 5 pigs to induce HFpEF3. cMRE was performed at baseline (Bx, prior to surgery), month 1 (M1) and month 2 (M2) on a 1.5T MRI scanner (Avanto, Siemens Healthcare, Erlangen, Germany). cMRE imaging parameters includes: TR/TE = 12.5/9.71 ms; FOV = 384 × 384 mm2; Resolution = 3 × 3 × 8 mm3; Flip angle = 15°; GRAPPA = 2; Mechanical frequency = 80 Hz; Encoding frequency = 160 Hz; Phase offsets = 4. Images were masked to extract the LV and estimate cMRE-derived LV MS across the cardiac cycle using 3D local frequency estimation inversion algorithm at each time point. End- systolic (ES) and diastolic (ED) MS were correlated to the corresponding ES and ED pressures obtained using LV catheterization. Results: Fig 1 demonstrates that cMRE-derived stiffness increases with disease progression from Bx to M1 to M2 throughout the cardiac cycle indicating that HFpEF causes an elevation in LV MS. Furthermore, ES MS is significantly higher (p-value < 0.001) than ED MS at all-time points. Fig 2 shows moderate correlation between ES and ED MS and corresponding pressure from LV catheterization with a R2 value of 0.4. Conclusions: Our result demonstrates that cMRE-derived MS increases with disease progression in HFpEF porcine model thereby indicating the scope of using cMRE as a diagnostic tool to diagnose HFpEF condition. (Figure Presented).