Neuroprotective effect of the n-hexane extracts of Laurus nobilis L. in models of Parkinson's disease

Abstract

Free radical scavenging and antioxidants have attracted attention as a way to prevent the progression of Parkinson's disease (PD). This study was carried out to investigate the effects of n-hexane fraction from Laurus nobilis L. (Lauraceae) leaves (HFL) on dopamine (DA)-induced intracellular reactive oxygen species (ROS) production and apoptosis in human neuroblastoma SH-SY5Y cells. Compared with apomorphine (APO, IC50=18.1 μM) as a positive control, the HFL IC50 value for DA-induced apoptosis was 3.0 μg/ml, and two major compounds from HFL, costunolide and dehydrocostus lactone, were 7.3 μM and 3.6 μM, respectively. HFL and these major compounds signifi cantly inhibited ROS generation in DA-induced SH-SY5Y cells. A rodent 6-hydroxydopamine (6-OHDA) model of PD was employed to investigate the potential neuroprotective effects of HFL in vivo. 6-OHDA was injected into the substantia nigra of young adult rats and an immunohistochemical analysis was conducted to quantitate the tyrosine hydroxylase (TH)-positive neurons. HFL signifi cantly inhibited 6-OHDA-induced TH-positive cell loss in the substantia nigra and also reduced DA induced α-synuclein (SYN) formation in SH-SY5Y cells. These results indicate that HFL may have neuroprotective effects against DA-induced in vitro and in vivo models of PD. © 2011 The Korean Society of Applied Pharmacology.