Effects of whole blood storage on results for glycosylated hemoglobin as measured by ion-exchange chromatography, affinity chromatography, and colorimetry

Abstract

After storage of whole blood at either 4 or 20°C, results for glycosylated hemoglobin by ion-exchange chromatography ('high-performance' liquid and mini-column chromatography), thiobarbituric acid colorimetry, and affinity chromatography were compared. At 4°C, all methods gave acceptable results for samples stored for as long as a week. At 20°C, the colorimetric and affinity methods also showed sample stability for a week or more. The ion-exchange methods were associated with a marked increase in values for glycosylated hemoglobin after a few days of storage. Evidently, care in details of sample collection and handling is especially important for ion-exchange methods, and the colorimetric and affinity methods have advantages over ion exchange in situations where long delays between sample collection and assay are unavoidable.