Genetic diversity and structure of Crupina vulgaris (common crupina): a noxious rangeland weed of the western United States

Abstract

Common crupina (Crupina vulgaris) is a federal noxious weed in the western USA that is currently the target of a classical biological control programme using the fungus Ramularia crupinae. We first identified and determined the location of populations of the two varieties of common crupina in the western United States and assessed the pattern of genetic diversity and structure of these populations. We found seven AFLP (Amplified Fragment Length Polymorphism) genotypes for 326 plants in 17 populations. AFLP genotypes correlated with two taxa, either C. vulgaris var. vulgaris or C. vulgaris var. brachypappa. This annual species is outcrossing, but relies on selfing when pollination does not occur, which may explain why less than 1% of the genetic variation is within populations. We found strong population genetic structuring and can typically predict genotype or variety for a given location. Researchers and managers will be able to predict and survey for differential efficacy of R. crupinae on the different genotypes and varieties during initial biological control field releases, thereby increasing the likelihood of successful biocontrol establishment and impact.