Selective purification of tartrate-inhibitable acid phosphatases: rapid and efficient purification (to homogeneity) of human and canine prostatic acid phosphatases

73Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.
Get full text

Abstract

We describe the synthesis of a long-chain monoamide derivative of L(+)-tartaric acid and its attachment to Sepharose 4B. Procedures are then described for use of this material in purifying human prostatic and (for the first time) canine prostatic acid phosphatases to constant specific activity and electrophoretic homogeneity. Depending on sample size, such purification is possible in one step, and is clearly faster and more efficient than are previously described methods. These materials and procedures have significant potential in studies of the comparative biochemistry and clinical chemistry of tartrate-inhibitable acid phosphatases.

Cite

CITATION STYLE

APA

Van Etten, R. L., & Saini, M. S. (1978). Selective purification of tartrate-inhibitable acid phosphatases: rapid and efficient purification (to homogeneity) of human and canine prostatic acid phosphatases. Clinical Chemistry, 24(9), 1525–1530. https://doi.org/10.1093/clinchem/24.9.1525

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free