A CRISPR/Cas9 system adapted for gene editing in marine algae

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Abstract

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.

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Nymark, M., Sharma, A. K., Sparstad, T., Bones, A. M., & Winge, P. (2016). A CRISPR/Cas9 system adapted for gene editing in marine algae. Scientific Reports, 6. https://doi.org/10.1038/srep24951

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