PHYTOCHEMICAL, ANTIFUNGAL AND ANTIOXIDANT PROPERTIES OF LEAF EXTRACTS OF THULASIVETTILA AN INDIGENOUS CULTIVAR OF PIPER BETLE L. IN KERALA.

Abstract

In the present study, a fine systematic investigation of antioxidant potential of Thulasivettila cultivar of Piper betle L. in Kerala region has been carried out. The results clearly showed chloroform and methanol leaf extracts of this cultivar recorded significant antifungal activity.Phytochemical analysis revealed that phenols, flavonoids, alkaloids, tannins, terpenoids, glycosides are present in Hexane, Chloroform, and Methanol extracts. The antioxidant property of Hexane, Chloroform, and Methanol extracts of Thulasivettila were evaluated by Diphenyl picrynl hydrazyl (DPPH) assay. The extracts of the plant at concentrations of 12.5, 25, 50, 100, and 200µg/mL were studied. The Chloroform extract clearly indicate the antioxidant activity of the plant compared with other extracts. The IC 50 values were calculated and compared with standard ascorbic acid. The results indicated that the chloroform extract exhibited good scavenging capacity.