The Amino Acid Sequence of Viscotoxin B from the European Mistletoe (Viscum album L, Loranthaceae)

Abstract

Viscotoxin B from Viscum album has been oxidized with performic acid and digested with trypsin and chymotrypsin. The resulting peptides have been separated by ion exchange chromatography and subjected to amino acid sequence determination by Edman degradation. Free lysine, six tryptic and three chymotryptic fragments are primary digestion products and permit deduction of the following amino acid sequence for the 46 amino acids of viscotoxin B: (Formula Presented.) This sequence is very similar to the amino acid sequence of viscotoxin A3 from the same plant. Copyright © 1971, Wiley Blackwell. All rights reserved