Seasonal lake surface water temperature trends reflected by heterocyst glycolipid-based molecular thermometers

Abstract

It has been demonstrated that the relative distribution of heterocyst glycolipids (HGs) in cultures of N2-fixing heterocystous cyanobacteria is largely controlled by growth temperature, suggesting a potential use of these components in paleoenvironmental studies. Here, we investigated the effect of environmental parameters (e.g., surface water temperatures, oxygen concentrations and pH) on the distribution of HGs in a natural system using water column filtrates collected from Lake Schreventeich (Kiel, Germany) from late July to the end of October 2013. HPLC-ESI/MS (high-performance liquid chromatography coupled to electrospray ionization-mass spectrometry) analysis revealed a dominance of 1-(O-hexose)-3,25-hexacosanediols (HG 26 diols) and 1-(O-hexose)-3-keto-25-hexacosanol (HG 26 keto-ol) in the solvent-extracted water column filtrates, which were accompanied by minor abundances of 1-(O-hexose)-3,27-octacosanediol (HG 28 diol) and 1-(O-hexose)-3-keto-27-octacosanol (HG 28 keto-ol) as well as 1-(O-hexose)-3,25,27-octacosanetriol (HG 28 triol) and 1-(O-hexose)-3-keto-25,27-octacosanediol (HG 28 keto-diol). Fractional abundances of alcoholic and ketonic HGs generally showed strong linear correlations with surface water temperatures and no or only weak linear correlations with both oxygen concentrations and pH. Changes in the distribution of the most abundant diol and keto-ol (e.g., HG 26 diol and HG 26 keto-ol) were quantitatively expressed as the HDI 26 (heterocyst diol index of 26 carbon atoms) with values of this index ranging from 0.89 in mid-August to 0.66 in mid-October. An average HDI 26 value of 0.79, which translates into a calculated surface water temperature of 15.8 ± 0.3 °C, was obtained from surface sediments collected from Lake Schreventeich. This temperature - and temperatures obtained from other HG indices (e.g., HDI 28 and HTI 28) - is similar to the one measured during maximum cyanobacterial productivity in early to mid-September and suggests that HGs preserved in the sediment record of Lake Schreventeich reflect summer surface water temperatures. As N 2 -fixing heterocystous cyanobacteria are widespread in present-day freshwater and brackish environments, we conclude that the distribution of HGs in sediments may allow for the reconstruction of surface water temperatures of modern and potentially ancient lacustrine settings.