Ekstraksi Dan Fraksinasi Betakaroten dari Minyak Sawit Mentah untuk Suplemen Pro Vitamin A

Abstract

Crude palm oil containd carotenoids around 500-700 ppm form of α-, β-, γ-carotene and lycopene. Compound β--karoten beneficial to supplement pro vitamins A and this compound may be separated from karotenoid other in crude palm oil by means of extraction and fractination. The purpose of this research is to know the composition of the solvent extraction of carotenoids in the best of crude palm oil and find out the type of solvent in oil β-carotene carotenoid extract from crude palm oil. A solvent used in extraction β-karoten is dietil eter-aseton with a composition: 1: 3 (A), 2: 4 (B) and 3: 5 (C). Fractionate β -karoten committed using column chromatography with a solvent n-hexan: butanol and identification β-karoten done with chromatography thin layers. The results showed that the solvent composition 1: 3 can yield the highest β-carotene which is equal to 584.383. Fractionate β -karoten of an extract carotenoid msm succeeded is conducted with a composition of a solvent n-hexan and butanol (98,08: 02 ). Analysis serious TLC indicating the existence of β-karoten with Rf 0,35.