Synbiotic and nutrients supplement improved of Secretory Immunoglobulin A (sIgA) in treated pulmonary tuberculosis patients

Abstract

Basically, bacterial populations in the gut ecosystem of healthy people who eat a balance diet are generally stable. Used of anti-tuberculosis drugs that consist of several antibiotics was alterating of the human gut microbiota. To analyze efficacy of milk-based protein supplement, synbiotic and micronutrients supplements on maintaining of microflora balance and increasing of sIgA level of treated pulmonary tuberculosis patient. A double-blind randomized treatment-control trial design. Data of microflora population of Lactobacillus sp. and Bifidobacterium sp. in human fecal was enumerated using plate count method and counting chambers method. Determination of secretory IgA (sIgA) in saliva was using sIgA ELISA Kit. Both subjects of treatment and control groups got same standard package of anti-tuberculosis drugs and MBP supplementation, except of treatment subjects were given synbiotic and micronutrients. Wilcoxon signed ranks test and Mann-WhitneyU-test. After first month of intervention, total colony of Lactobacillus sp. and Bifidobacterium sp. were higher in treatment group than control groups (p < 0.05). sIgA concentration in saliva of treatment group did not changes (p > 0.05) in all phase, contrary in the control group sIgA titers after 6 mo was declined (p < 0.05). There was positive effect of these supplements to maintain balance of gut microflora and stimulating sIgA secretion during TB chemotherapy.