Screening of antiangiogenie activity of some tropical plants by rat aorta ring assay

Abstract

Angiogenesis is essential for the growth and metastasis of most solid malignancies. Accordingly, tumor angiogenesis is an important pharmacological target for cancer prevention and treatment. This study was conducted to study antiangiogenic activity of some tropical plants. Seven plants were extracted successively with n-hexane and methanol to prepare 18 extracts and antiangiogenic properties of the extracts were studied by rat aorta ring assay. Nine extracts showed more than 50% inhibition of the blood vessels outgrowth from the primary tissue explants. The MTT assay was used to study the cytotoxic activities of extracts with more than 50% inhibition. The Human Umbilical Vein Endothelial Cells was used as a test cell line versus two human colon cancer cell lines HCT-116 and HT-29, two human breast cancer cell lines MCF-7 and T47D and one hepatocarcinoma cell line HepG-2. The selectivity index on Human Umbilical Vein Endothelial Cells was calculated. The selectivity index results indicated antiangiogenic activities of three plants Parkia speciosa, Syzygium campanulatum and Sandoricum koetjape. The results presented in this research article make these plants good candidates for further studies to purify the active compounds and to study in vivo antiangiogenic activity. © 2009 Asian Network for Scientific Information.