THE EFFECT OF Δ1‐TETRAHYDROCANNABINOL ON THE RELEASE OF [3H]‐(‐)‐NORADRENALINE FROM THE ISOLATED VAS DEFERENS OF THE RAT

Abstract

The amount of tritium released upon transmural stimulation of the isolated vas of the rat incubated with [3H]‐Δ1‐tetrahydrocannabinol (THC) (2.8 μM) was significantly greater than the non‐stimulated overflow. There was no difference between non‐stimulated overflow and the effect of transmural stimulation of vasa equilibrated with [14C]‐sorbitol (6.5 mg/ml). The difference between non‐stimulated and stimulated efflux from vasa equilibrated with [3H]‐Δ1‐THC was abolished in rats pretreated for 24 h with 6‐hydroxydopamine (250 mg/kg). The amount of tritium released upon transmural stimulation of the vas incubated with [3H]‐noradrenaline (6 μm) was significantly greater than the non‐stimulated overflow. Δ1‐THC (560 Nm and 14 μm) caused a significant dose‐dependent reduction of both non‐stimulated and stimulated tritium efflux, especially the latter. 1974 British Pharmacological Society