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Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

Journal of Bacteriology
DOI: 10.1128/jb.176.21.6764-6768.1994
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Abstract

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

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Tsinoremas, N. F., Kutach, A. K., Strayer, C. A., & Golden, S. S. (1994). Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination. Journal of Bacteriology. American Society for Microbiology. https://doi.org/10.1128/jb.176.21.6764-6768.1994

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