Effects of hydroxyl, carboxyl, and amino functionalized carbon nanotubes on the functional diversity of microbial community in riverine sediment

Abstract

Nowadays, more and more attention is focused on the environmental harm brought by the wide production and use of carbon nanotubes. In this study, the metabolic function of sediment microbial community was investigated after unfunctionalized or functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated. The surface functional groups on the studied functionalized MWCNTs in this work were hydroxyl, carboxyl, and amino, respectively. The metabolic functional diversity was determined by Biolog EcoPlates after one-month exposure to MWCNTs. Incorporating 0.5 wt% amino functionalized MWCNTs significantly decreased the microbial activity and diversity, and all types of MWCNTs caused great inhibition on the microbial metabolism at the dosage of 2.0 wt%. The sediment microbes preferred polymers and amino acids. Principal component and similarity analysis indicated that the microbial carbon metabolism was more affected by the MWCNT dosage compared with the functionalization, and 2.0 wt% amino functionalized MWCNTs made the greatest difference in metabolic function of sediment microbial community. These consequences may help to assess the environmental risks of MWCNTs from the aspect of ecological relevance of sediment microbial community.