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Protein Determination by the Biuret Method

  • Nowotny A
Springer Berlin Heidelberg, (1979), 168-169
DOI: 10.1007/978-3-642-67356-6_50
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Abstract

The biuret reaction, used for the quantitative determination of proteins, is based upon the formation of a copper chelate with the peptide bonds of the protein at alkaline pH. In this complicated reaction one copper atom forms a complex with four peptide nitrogens.

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Nowotny, A. (1979). Protein Determination by the Biuret Method. In Basic Exercises in Immunochemistry (pp. 168–169). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_50

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