Detection of tet gene in multidrug-resistant salmonella spp. Isolates from layers and broiler breeders

Abstract

The present study was conducted with the following aims: (i) To detect the multidrug-resistant Salmonella spp. isolates recovered from faeces, litter and drinking water in layers and broiler breeders’ farms and (ii) to carried out the detection of the tet gene. A total of 21 Salmonella isolates were subjected to Polymerase Chain Reaction (PCR) assay to determine the presence of tet gene. Out of 21 isolates, 14 (66.7%) and 7 (33.3%) were found positive for tet(A) and tet(B), respectively. In antimicrobial susceptibility tests, the Salmonella isolates showed resistance to tetracycline, oxytetracycline, ampicillin, nalidixic acid, enrofloxacin, gentamicin and chloramphenicol. It can be concluded that the high prevalence of the tet gene indicates a high potential of Salmonella isolates for horizontal transmission of tetracycline resistance genes.