Cytotoxic Activity of Crude Extracts and Fractions from Irvingia malayana

Abstract

Irvingia malayana has been utilized for traditional medicine in West Kalimantan, Indonesia.The aim of this research is to detect cytotoxic activity of extract of Irvingia malayana. The extraction with methanol and partition by variated eluen polarity. This extraction produced methanol extract, n-hexane fraction, methylene chloride fraction, ethylacetate fraction and methanol fraction. Cytotoxic test using MTT assay method was done to each extract and fraction. Cell viability profiles produced from MTT assay showed that methanolic extract , the other fractions (n-hexane, metylen chloride, ethylacetate and methanol) from bark of Irvingia malayana decrease HeLa cells viability compare to control cells in the concentration dependent manners with IC 50 of 59, 92, 30, 22, and 33 μg/mL respectively. The strongest cytotoxic activity was showed by ethylacetate fraction of bark of Irvingia malayana, but methylen chloride fraction of root of Irvingia malayana also potencial to find bioactive compound against Hela cell line.