AdoR-1 (Adenosine Receptor) Contributes to Protection against Paraquat-Induced Oxidative Stress in Caenorhabditis elegans

Abstract

AdoR-1. The single adenosine receptor homolog in Caenorhabditis elegans, which belongs t. The superfamily of G-protein coupled receptors (GPCRs), mediates most o. The physiological effects of extracellular adenosine. Adenosine has been proved to improv. The survival rate of C. elegans in oxidative stress conditions. However. The potential mechanism of adenosine's protective effect against oxidative stress via AdoR-1 has not been studied. In this study, C. elegans were divided into three groups: two groups with paraquat treatment, one i. The presence and one i. The absence of adenosine, and an untreated control group. Results indicate that many differentially expressed genes were found to be enriched significantly in neural-related signaling pathways among transcriptome data of three groups. Further gene network analysis showed that some important genes well known to be involved in promotin. The acetylcholine release pathway, such as dop-1, egl-30, and unc-13, and those involved in promotin. The neuropeptide release pathway, such as kin-1, were upregulated by paraquat induction but downregulated after adenosine treatment. Meanwhile, a completely opposite trend was observed fo. The goa-1 gene that inhibit. The acetylcholine-release and neuropeptide-release pathway. Additionally, some biochemical assays including SOD, GSSG, GSH, and AChE were measured to identif. The potential protection of adenosine against oxidative stress between wild-type strain N2 and ador-1 gene knockout strain EG6890. Conclusively, our study revealed series of adenosine receptor-mediated genes in C. elegans that might act as regulators of paraquat-induced oxidative stress and may indicate adenosine's promising protective effects.