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Feeder-free, Xeno-free Generation of Cortical Spheroids From Human Pluripotent Stem Cells

  • Pasca S
  • Yoon S
  • Pasca S
Protocol Exchange (2018)
DOI: 10.1038/protex.2018.123
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Abstract

Here, we show the generation of 3D human cortical spheroids (hCS) from human induced pluripotent stem cells (hiPSC) maintained in feeder-free, xeno-free conditions. This protocol describes hiPSC maintenance, generation of 3D cell aggregates in microwells, and cortical differentiation into hCS

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APA

Pasca, S., Yoon, S.-J., & Pasca, S. (2018). Feeder-free, Xeno-free Generation of Cortical Spheroids From Human Pluripotent Stem Cells. Protocol Exchange. https://doi.org/10.1038/protex.2018.123

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