Effects of the standardized extract of Centella asiatica ECa233 on hepatic Phase II drug-metabolizing enzymes in rats

Abstract

Introduction: The standardized Effects of the standardized extract (ECa233) was shown to possess neuroprotective and wound healing effects. Purpose of the Study: The purpose of this study was to investigate effect of ECa233 on the activities of Phase II drug-metabolizing enzymes including uridine 5’-diphosphoglucuronosyltransferase (UDPGT), sulfotransferase (SULT), glutathione S-transferase (GST), and NAD(P)H quinone oxidoreductase (NQOR) in rats. Method: ECa233 was orally given to both male and female Wistar rats at doses of 10, 100, and 1000 mg/kg/d for 90 days. Result and Conclusion: The results showed that all regimens of ECa233 did not affect the activities of UDPGT in both male and female rats. Furthermore, in line with our previous study in male rats, it was found that neither the activities of GST nor NQOR in female rats was modulated by ECa233. However, the activity of SULT, which had been found to significantly decrease in male rats, was unaltered in female rats. The obtained results provide the proof of evidence of safety of ECa233 on Phase II drug-metabolizing enzymes in animals, then interpretation of these data from animals to human remains to be further investigated.