Identification of bitter constituents from angelica keiskei KOIDZUMI

Abstract

Laserpitin (LAS), isolaserpitin (ILA) and (8S,9R)-8-angeloxy-8,9-dihyrooroselol (DHO) were identified as bitter constituents from Angelica (A.) keiskei KOIDZUMI, which is becoming popular as vegetables and dietary supplements in Japan. A yellow juice from the aerial parts of 140 samplesof A. keiskei could be grouped into 4 types (Type A~D) according to the HPLC profiles with the peaks of bitter constituents. Type A was from the Hachijyo island strain, which detected LAS and ISO, and Type B was from the Oshima island strain, which only detected DHO. The LAS, ILA and DHO contents were 0.066~0.092%, 0.040~ 0.068% and 0.064~0.279%, respectively. © 2009 The Japan Society for Analytical Chemistry.