Total Flavonoids Content of Polar Extracts of Cayratia trifolia Leaves

Abstract

Cayratia trifolia leaves contain flavonoids compound that can dissolve well in polar solvents. This study aimed to evaluate the total flavonoids content of polar extracts which extracted sequentially from C. trifolia leaves. The dried C. trifolia leaves were macerated continually using n-hexane, ethyl acetate, and ethanolic solvent. The total flavonoids content was determined using spectrophotometric methods with a AlCl3 reagent. TLC chromatogram of the extracts were evaluate using chloroform: aceton: formic acid (10:2:1) as a mobile phase and silica gel F254 as a stationary phase. The citroborate and ammonia vapour were used as detection spray reagents. The highest total flavonoids content (27.95?0.62 mgQE/g) were found in 70% ethanol extract of C. trifolia leaves compared to ethyl acetate extract (17.98 ? 0.89 mgQE/g). The ethanolic extract is found six compound spots, while the ethyl acetate extract found four compound spots which were thought to be a flavonoid group. Thus, it can be concluded that the different polar extracting solvents are able to attract flavonoid compounds with different levels. The flavonoids in C. trifolia extract need to be separated to determine the types.