Second harmonic generation microscopy of otoconia

  • Brittain K
  • Harvey M
  • Cisek R
  • et al.
13Citations
Citations of this article
13Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The origin of second harmonic generation (SHG) signal in otoconia was investigated. SHG signal intensity from otoconia was compared to pure calcite crystals, given calcite is the primary component of otoconia and is known to emit surface SHG. The SHG intensity from calcite was found to be ∼41× weaker than the SHG intensity from otoconia signifying that the SHG signal from otoconia is likely generated from the organic matrix. Furthermore, the SHG intensity from otoconia increased when treated with a chelating agent known to dissolve calcite which confirms that calcite is not the source of SHG. Additionally, polarization-resolved SHG microscopy imaging revealed that the arrangement of the SHG emitters is radial and can form highly ordered domains.

Cite

CITATION STYLE

APA

Brittain, K., Harvey, M., Cisek, R., Pillai, S., Christie, S. D., & Tokarz, D. (2022). Second harmonic generation microscopy of otoconia. Biomedical Optics Express, 13(6), 3593. https://doi.org/10.1364/boe.457967

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free