Micropropagation of Kalanchoe tubiflora (Harvey) Hamet

Abstract

Kalanchoe tubiflora (Harvey) Hamet is a succulent plant native to Madagascar. Due to its unique morphology, it is a popular ornamental species. However, owing to some valuable metabolites it can also be used in tumor suppression. The present work describes a simple propagation method of K. tubiflora. The stem fragments of K. tubiflora were disinfected with the following sodium hypochlorite concentrations: 0.2% (for 10 or 20 min), 0.4% (10 or 20 min) and 0.6% (10 or 20 min). Next the single-node explants were inoculated on a hormone-free MS medium. The produced plants were transferred to soil and grown in a greenhouse. The applied disinfection procedures were successful. The regeneration of propagules was observed. The average number of new microshoots produced from one explant varied from about 20 to even 52. All microshoots regenerated adventitious roots without transferring them to a rooting medium. The plantlets were successfully acclimatized and continued their growth in the greenhouse. (English) [ABSTRACT FROM AUTHOR]