Optimising High-Pressure Processing (HPP) for Optimal Total Phenolic and Flavonoid Content in Kelulut (Stingless Bee) Honey

Abstract

High-pressure processing (HPP) has the potential to enhance the total phenolic and flavonoid content (TPC and TFC) in Kelulut honey (KH). However, KH’s inherent variability possesses challenges in optimising these phytochemicals. This study hence explores the optimisation of KH through HPP, focusing on TPC and TFC. The Face-centered Central Composite Design (FC-CCD), involving two factors with three levels each (pressure of 200, 400, and 600 MPa, and time of 5, 10, and 15 min), was applied to investigate two response variables (total phenolic and flavonoid content). The optimisation identified the optimal parameters as 200 MPa for 15 min, with a desirability of 0.986, indicating precise modeling. Compared to other pressures (400 and 600 MPa) at common processing time (15 minutes), 200 MPa recorded higher TPC and TFC with differences of 25.71% to 30.92% and 13.78% to 14.19%, respectively. A verification step revealed that HPP-KH at 200 MPa for 15 minutes yielded a TPC of 15.278 ± 0.525 mg GAE/100 g and a TFC of 38.274 ± 1.980 mg RE/100 g, indicating the precise accuracy of the quadratic modeling. However, this discovery contradicts the consensus that pressures above 500 MPa increase TPC and TFC. Consequently, it underscores the need for tailored high-pressure strategies in KH processing, offering essential insights for industry applications and further research endeavors.