Expression patterns of cytokines, p53 and nitric oxide synthase isoenzymes in corpora lutea of pseudopregnant rabbits during spontaneous luteolysis

28Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

The gene expressions for macrophage chemoattractant protein-1 (MCP-1), interleukin (IL)-1β, IL-2 and p53 were examined by semi-quantitative RT-PCR in corpora lutea (CL) of rabbits during spontaneous luteolysis at days 13, 15, 18 and 22 of pseudopregnancy. In the same luteal tissue, total activity of nitric oxide (NO) synthase (NOS) and genes for both endothelial (eNOS) and inducible (iNOS) isoforms were also analysed. From day 13 to IS, MCP-1 and IL-1β mRNA levels rose (P ≤ 0.01) almost 2-fold, and the transcript for p53 almost 8-fold, but then all dropped (P ≤ 0.05) from day 18 onward. IL-2 mRNA abundance was higher (P ≤ 0.01) on day 13 and then gradually declined. During luteolysis, eNOS mRNA decreased 40% (P ≤ 0.05) by day 15, but thereafter remained unchanged, while iNOS mRNA was barely detectable and did not show any clear age-related pattern throughout the late luteal stages. Total NOS activity progressively increased (P ≤ 0.01) from day 13 to 18 of pseudopregnancy and then dropped to the lowest (P ≤ 0.01) levels on day 22. Luteal progesterone content also declined during CL regression from 411 to 17 pg/mg found on days 13 and 22 respectively, in parallel with the decrease in blood progesterone concentrations. These data further support a physiological role of NO as modulator of luteal demise in rabbits. Locally, luteal cytokines may be involved in the up-regulation of NOS activity, while downstream NO may inhibit steroroidogenesis and induce expression of p53 gene after removal of the protective action of progesterone. © 2004 Society for Reproduction and Fertility.

References Powered by Scopus

NO at work

1523Citations
N/AReaders
Get full text

Bcl-2 family proteins

957Citations
N/AReaders
Get full text

Mechanisms controlling the function and life span of the corpus luteum

827Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Blood flow: A key regulatory component of corpus luteum function in the cow

122Citations
N/AReaders
Get full text

Structure, Function, and Regulation of the Corpus Luteum

43Citations
N/AReaders
Get full text

Expression of luteal estrogen receptor, interleukin-1, and apoptosis-associated genes after PGF <inf>2α</inf> administration in rabbits at different stages of pseudopregnancy

35Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Boiti, C., Guelfi, G., Zerani, M., Zampini, D., Brecchia, G., & Gobbetti, A. (2004). Expression patterns of cytokines, p53 and nitric oxide synthase isoenzymes in corpora lutea of pseudopregnant rabbits during spontaneous luteolysis. Reproduction, 127(2), 229–238. https://doi.org/10.1530/rep.1.00083

Readers over time

‘13‘15‘16‘17‘18‘19‘20‘21‘22‘23‘2400.751.52.253

Readers' Seniority

Tooltip

Professor / Associate Prof. 4

33%

PhD / Post grad / Masters / Doc 4

33%

Researcher 4

33%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 5

50%

Veterinary Science and Veterinary Medic... 4

40%

Medicine and Dentistry 1

10%

Save time finding and organizing research with Mendeley

Sign up for free
0