Azospirillum lipoferum tRNAs: Fractionation and identification of tRNA species and the nucleotide sequence of tRNAAsn (QUU)

Abstract

Transfer RNAs of Azospirillum lipoferum were separated by two- dimensional gel electrophoresis and identified by aminoacylation. Thirty-six tRNA spots were resolved by this technique and twenty-six tRNA species have been identified. There are five tRNAs for Leu, four for Val, three for Pro, two each for Arg, Ile, Lys and Tyr, and one each for Ala, Asp, His, Phe, Ser and Thr. The tRNAAsn (QUU) was purified and its nucleotide sequence was determined. The A. lipoferum tRNAAsn(QUU) is 92% similar to B. subtilis tRNAAsn gene and two hypermodified nucleosides, queuosine (Q) and N-(9-β-D Ribofuranosylpurine-6-YL) - carbamoyl)- threonine (t6A) are present in this tRNA.