First record of Hippa admirabilis Thallwitz, 1891 (Crustacea: Decapoda: Hippidae) from Tomini Bay, Province Gorontalo, Indonesia confirmed by DNA barcoding

Abstract

Hippoid crab is a crustacean group which inhabits sandy beach in tropical and subtropical areas. There are seven species of hippoid crab has been reported from Indonesia so far, i.e. six from family Hippidae and one from family Albuneidae. One of them is Hippa admirabilis Thalwittz, 1891. This species is first record from Tomini Bay, province in our study. The Specimens were collected from Tomini Bay in 2016. To confirm the species, we used CO1 gene as DNA barcoding. The DNA was extracted from the muscle tissues. The upper part of CO1 gene was amplified using Primer AF215-216. The CO1 gene that we retrieved was 634 bp. The samples from our study have about 99.8% similarity with the sequence of Hippa admirabilis in Genebank with the accession number is KR047031.1. Based on morphological and molecular data, our samples are confirmed as H. admirabilis.