IDENTIFICATION OF DIGESTIVE ENZYME OF Anguilla bicolor bicolor DURING SEED EEL PHASE IN CONTROLLED CONTAINER

Abstract

The main constraint for Indonesian shortfin eel aquaculture is high mortality rate for glass eel to elver phase. This aim of study was to determine the activity pattern of digestive enzymes (protease, amylase and lipase) during seed eel to elver phase, i.e. day 1-71 of rearing period. Indonesian shortfin eel (Anguilla bicolor bicolor) during seed eel phase was reared in aquarium with aeration and filtration systems and fed with natural feeds (Artemia nauplii, Daphnia and silk worms). Enzyme activity samplings were carried out at day 1, 14, 28, 42, 56 and 71 of rearing period in aquarium. Measurement data were descriptive-quantitatively analyzed and presented in graphs. The study results indicated that 1) activities of protease, lipase and amylase were starting to be detected since day 1 with small-scaled activity for protease and large-scaled activity for lipase and amylase and 2) protease activity increased for 1-71 days-seed eel, amylase activity decreased at day 42 of rearing and re-increased at day 56 to 71 and lipase activity continuously decreased since day 1 to 71. The highest protease was detected at day 71, while the highest amylase and lipase activities were at day 1.