Occurrence of rpoB mutations in isoniazid-Resistant but rifampin-Susceptible mycobacterium tuberculosis isolates from germany

Abstract

Four out of 143 phenotypically isoniazid-resistant but rifampin-susceptible Mycobacterium tuberculosis strains that were isolated from patients in Germany in 2011 had mutations in the rifampin resistance-determining region of rpoB. After performing drug susceptibility testing (DST) with two methods, the proportion method on Löwenstein-Jensen medium and using the Bactec 960 Mycobacteria Growth Indicator Tube system, we conclude that the two methods are equally reliable for phenotypic DST and MIC determination. © 2014, American Society for Microbiology. All Rights Reserved.