Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor

Abstract

Membranes less attractive to embryos were tested as a replacement for nylon screens to prevent adherence of somatic embryos, cell clusters and cells to different sites of the bioreactor, a feature considered undesirable in plant cell suspension cultures. The results showed that the loss of embryogenic cell-mass could be halved by using silicone or track membranes. For aeration purposes, these membranes are as satisfactory as nylon screens conventionally used in cell lift impellers.