Elucidating Genotypic Variation in Quinoa via Multidimensional Agronomic, Physiological, and Biochemical Assessments

Abstract

Quinoa (Chenopodium quinoa Willd.) has emerged as a climate-resilient, nutrient-dense crop with increasing global popularity because of its adaptability under current environmental variations. To address the limited understanding of quinoa’s genotypic performance under local agro-environmental conditions, this study hypothesized that elite genotypes would exhibit significant variation in agronomic, physiological, and biochemical traits. This study aimed to elucidate genotypic variability among 23 elite quinoa lines under field conditions in Faisalabad, Pakistan, using a multidimensional framework that integrated phenological, physiological, biochemical, root developmental, and yield-related attributes. The results revealed that significant variation was observed across all measured parameters, highlighting the diverse adaptive strategies and functional capacities among the tested genotypes. More specifically, genotypes Q4, Q11, Q15, and Q126 demonstrated superior agronomic potential and canopy-level physiological efficiencies, including high biomass accumulation, low infrared canopy temperatures and sustained NDVI values. Moreover, Q9 and Q52 showed enhanced accumulation of antioxidant compounds such as phenolics and anthocyanins, suggesting potential for functional food applications and breeding program for improving these traits in high-yielding varieties. Furthermore, root trait analysis revealed Q15, Q24, and Q82 with well-developed root systems, suggesting efficient resource acquisition and sufficient support for above-ground plant parts. Moreover, principal component analysis further clarified genotype clustering based on trait synergistic effects. These findings support the use of multidimensional phenotyping to identify ideotypes with high yield potential, physiological efficiency and nutritional value. The study provides a foundational basis for quinoa improvement programs targeting climate adaptability and quality enhancement.