In vitro alterations of erythrocyte aggregation by action of Trichinella spiralis newborn larvae

Abstract

Possible changes in the erythrocyte membrane, by in vitro interaction with newborn larvae of T. spiralis (NL), were evaluated analyzing the alterations in erythrocyte aggregation by digital image analysis and laser transmission in a new optical chip aggregometer. NL were obtained from CBi mice infected with T. spiralis. RBCs samples from healthy donors where in vitro exposed to NL (concentration (3000±500) larvae/mL) to assess its effect on RBC aggregation. Individual cell Coefficient (CCA) and aggregation parameter (S) were calculated by digitally processingRBCaggregate images, indicating the amount and size of the erythrocyte aggregates present. Also, size distribution of aggregate was analyzed. Kinetic aggregation parameters (Amp750 and t1/2) were calculated with a new optical chip aggregometer. Results show significant alterations in erythrocyte aggregability due the in vitro action of T. spiralis larvae increasing incubation time. These results are possibly related to the loss of surface sialic acid as it is captured by NL. Obtained results suggest thatNLcould produce hemorheological alterations in the host, which could be related to thrombosis and anemia reported in some patients with trichinosis.