Terriglobus tenax sp. nov., an exopolysaccharideproducing acidobacterium isolated from rhizosphere soil of a medicinal plant

Abstract

An exopolysaccharide-producing bacterium, designated strain DRP 35T, was isolated from the rhizosphere soil of a medicinal herb, Angelica sinensis, at Geumsan in Korea. Cells were Gramstaining- negative, non-motile, catalase-positive and oxidase-negative short rods. The isolate grew aerobically from 15 to 45°C (optimum 30°C), pH 3.5-7.0 (optimum pH 5.0) and in the presence of 0-1.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain DRP 35T belongs to the genus Terriglobus in the phylum Acidobacteria with a sequence similarity of 97.2% and 97.0% to Terriglobus saanensis SP1PR4T and Terriglobus roseus KBS63T, respectively. The genomic DNA G+C content was 62.1 mol%. DNA-DNA relatedness between strain DRP 35T and the type strains of the other species of the genus Terriglobus, T. saanensis SP1PR4T and T. roseus KBS63T, were 24.6 and 17.2 %, respectively. The predominant menaquinone was MK-8. Major fatty acids were iso-C15: 0, C16: 1ω7c and C16: 0. The polar lipids were phosphatidylethanolamine, unidentified aminophospholipid and unknown phospholipids. On the basis of polyphasic analysis from this study, strain DRP 35T represents a novel species of the genus Terriglobus for which the name Terriglobus tenax sp. nov. is proposed. The type strain is DRP 35T (5KACC 16474T5NBRC 109677T). © 2014 IUMS.