VALIDATION METHODOLOGY FOR IDENTIFICATION AND MEASUREMENT OF PHENOLIC COMPOUNDS IN OIL REFINERY EFFLUENT BY HPLC

Abstract

The present work established a method, using High Performance Liquid Chromatography (HPLC) analysis, for the identification and quantification of five phenolic compounds. Chromatographic separation was performed by a reversed phase C18 column with a mobile phase consisting of methanol and aqueous 0.1% phosphoric acid in a ratio (10:90). The calibration curves showed excellent coefficients of determination (R 2 ≥ 0.99) within the concentrations tested. The recoveries were 84.09% to 99.98% for spiked samples. The method can be adapted for the detection of phenolic compounds, which can be applied to monitor the effluent discharged into the environment.