Crystal structure of an archaeal DNA sliding clamp: Proliferating cell nuclear antigen from Pyrococcus furiosus

Abstract

The proliferating cell nuclear antigen (PCNA) is now recognized as one of the key proteins in DNA metabolic events because of its direct interactions with many proteins involved in important cellular processes. We have determined the crystal structure of PCNA from a hyperthermophilic archaeon, Pyrococcus furiosus ( Pfu PCNA), at 2.1 Å resolution. Pfu PCNA forms a toroidal, ring‐shaped structure consisting of homotrimeric molecules, which is also observed in the PCNA crystals from human and yeast. The overall structure of Pfu PCNA is highly conserved with other PCNA proteins, as well as with the bacterial β clamp and the bacteriophage gp45. This result shows that the three‐dimensional structure of the sliding clamp is conserved in the three domains of life. Pfu PCNA has two remarkable features compared with the human and yeast PCNA molecules: it has more ion pairs and fewer intermolecular main chain hydrogen bonds. The former may contribute to the thermal stability of Pfu PCNA, and the latter may be the cause of the stimulatory effect of Pfu PCNA on the DNA synthesizing activity of P. furiosus DNA polymerases in the absence of the clamp loader replication factor C in vitro.