Abstract
Abstract: A monomeric sensor, TagRFP-23-Ultramarine (TR-23-U), for effector caspase-3 was obtained. The overlap integrals of new pairs of red fluorescent protein TagRFP with four chromoproteins were calculated. The monomeric state of the Ultramarine protein and the TR-23-U sensor was confirmed by gel filtration chromatography and dynamic light scattering. Incubation with caspase-3 showed the possibility of using the new fusion protein as a FRET sensor for apoptosis detection.
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Marynich, N. K., Granovsky, I. E., & Savitsky, A. P. (2022). New FRET Pairs of Fluorescent Proteins for In Vitro Caspase Activity Determination. Applied Biochemistry and Microbiology, 58(6), 738–743. https://doi.org/10.1134/S0003683822060084
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