Salmonella typhimurium mutants defective in the formate dehydrogenase linked to nitrate reductase

Abstract

Six fdn mutants of S. typhimurium defective in the formation of nitrate reductase-linked formate dehydrogenase (FDH(N)) but capable of producing both the hydrogenase-linked formate dehydrogenase (FDH(H)) and nitrate reductase were characterized. Results of phage P22 transduction experiments indicated that there may be three fdn genes located on the metE-metB chromosomal segment and distinct from all previously identified fdh and chl loci. All six FDH(H)+ FDH(N)- mutants were found to make FDH(N) enzyme protein which was indistinguishable from that of the wild type in electrophoretic studies. However, the results of the spectral studies indicated that all six mutants were defective in the anaerobic cytochrome b559 associated with FDH(N). All contained the cytochrome b559 associated with nitrate reductase in amounts equal to or greater than the wild type. The results of the transduction experiments also indicated that the metE-metB segment of the Salmonella chromosome resembles that of Escherichia coli more than was originally thought.